L-012化學(xué)發(fā)光探針
生化學(xué)用 for Biochemistry3116094040028274.png 規(guī)格含量 : 90.0+%（HPLC） 制造商 : FUJIFILM Wako Pure Chemical Corporation（富士膠片和光） 儲(chǔ)存條件：冷藏（干冰運(yùn)輸） CAS RN? : 143556-24-5 分子式 : C13H8ClN4NaO2 分子量 : 310.67 |  |
◆基本信息
L-012 是一種高靈敏度的化學(xué)發(fā)光（CHL）探針，比魯米諾（Luminol）試劑更有效。L-012 與多種類(lèi)型的活性氧反應(yīng)，這些活性氧來(lái)自人血液和口腔中激活的中性粒細(xì)胞或者大鼠腹膜腔。該產(chǎn)品可以用于有辣根過(guò)氧化物酶的ELISA 試劑盒，可提高反應(yīng)靈敏度。
結(jié)構(gòu)式
溶解及保存：蒸餾水溶解成20 mM/L（6.23 mg/mL）的水溶液，再用50 mM/L的Tris鹽酸緩沖液（pH 7.5）稀釋 至最終濃度為500 μM/L（有細(xì)胞的反應(yīng)體系）。另外，在沒(méi)細(xì)胞參與的反應(yīng)體系中時(shí)，建議使用終濃度為5~50 μM/L。母液冷凍保存，使用時(shí)再稀釋。
◆物性信息
外觀 | 白色~黃色，結(jié)晶~粉末 |
溶解性 | 可溶于水。 |
| 產(chǎn)品編號(hào) | 產(chǎn)品名稱(chēng) | 產(chǎn)品規(guī)格 | 產(chǎn)品等級(jí) |
| 120-04891 | L-012 L-012化學(xué)發(fā)光探針 | 100 mg | － |
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Q&A
Q： L-012能滲入細(xì)胞嗎?
A： 由于L-012對(duì)脂質(zhì)高度親和，它比luminol和lucigenin更能具有細(xì)胞滲透性。另一方面，ROS無(wú)限制地在胞外釋放。因此L-012被認(rèn)為也能
檢測(cè)胞外ROS。
Q： L-012的使用濃度如何?
A： 這取決于應(yīng)用和條件。
[體外] 培養(yǎng)細(xì)胞（EoL-1細(xì)胞）： 500 ~ 800 μmol/L.
全血和分離的中性粒細(xì)胞： 400 μmol/L
多形核中性粒細(xì)胞（PMNs）（由小鼠中分離）： 2 mmol/L
無(wú)細(xì)胞的樣品： 4 μmol/L, 15 μmol/L, and 50 μmol/L
[體內(nèi)] Balb/c 小鼠[皮下]： 20 ~ 80 μL of L-012(5mg/mL)
Balb/c 小鼠[腹膜內(nèi)]： 100 μL of L-012 (15mg/mL)
C57BL/10.Q (B10.Q) 小鼠 [腹膜內(nèi)]： 1 ~ 75 mg/kg
Q： 是否可用于動(dòng)物活體成像？
A： 如果所用的小動(dòng)物成像儀中有發(fā)光檢測(cè)探頭，就可以進(jìn)行檢測(cè)。以下是文獻(xiàn)報(bào)道過(guò)的儀器型號(hào)：
?IVIS 100 imaging systems
?Infinite M200 [Tecan]
?Luminescence Reader BLR-201 [Aloka]
?Beckman-Coulter DTX880 multimode plate reader [Beckman]
Q： 是否可用于固定細(xì)胞？
A： 目前尚無(wú)證據(jù)表明，活性氧（ROS）在細(xì)胞活性停止后仍然存在。普遍看法是認(rèn)為在細(xì)胞活性停止后（細(xì)胞死亡）ROS消失。固定組織或
細(xì)胞會(huì)停止細(xì)胞活性，因此不建議用于ROS檢測(cè)。
本品無(wú)需固定檢測(cè)對(duì)象即可檢測(cè)到ROS，使用方法包括但不限于將其添加到細(xì)胞培養(yǎng)上清液中或進(jìn)行動(dòng)物腹腔注射。
參考文獻(xiàn)
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